by "low E" I'm guessing you mean the 6th string, the thick one? I'd bet you've backed the saddle back as far as you can and it's still a bit sharp? I'd have to see the bridge assembly to see what you can do beyond that. Is there a coil spring surrounding the screw? Sometimes you can disassemble the saddle unit and remove the spring and reassemble it w/o the spring allowing you to adjust back that much more, however sometimes your adjustment screw begins to pinch the string inside the saddle cavity. If there is one. I'd really need to see it.
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