I use a tuner live, as tuning can be a nightmare in a busy club or pub.
I can get very close to tuning concert E or A by ear, but often use a reference note when I am about to record. I will use a tuner for that normally. After that I check the tuning by fretting the notes at the 5th fret, using harmonics from the 12th back to the 7th fret on the next string, , and playing a couple of stacked 5th interval shapes to see how they sit. When playing with a keyboard I will tune to that, or if playing in an open tuning I will make fine adjustments to the chord.
As I have gotten better I have found myself getting more and more annoyed with my guitars for not being in tune. Playing sax and trumpet has helped a lot on that regard.
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