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Originally Posted by Matty Ryan
I'm not sure if this is the right place to put this, but I'm having trouble with my A string. I've got a Squier Affinity Strat that still has the original strings on it. Its hardly been used. On my first day of practise, everything was fine. When I went back to it on the second day, the A string sounded flat. At least I think thats the term. I've got an electronic tuner on my effects pedal, and according to that all of the strings are tuned properly. So I tried that fifth fret method, and everything is fine apart from that A string. I can't see how I can get the low E and A strings to sound the same. My guitar isn't knackered is it? 
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I doubt the squire was set up before you bought it. Sounds like an intonation problem. Read UGBs post on intonating your guitar.