Did you use the Sounds and Audio Devices (in the Control Panel) to make sure the sound card that you were trying to use was selected?
It sounds like you have the right adapter (1/4" to 1/8") and the stereo input shouldn't make any difference.
The recording application also won't make any difference at this point. You should be able to hear your guitar with no recording applications open.
-tkr
'Cause I don't wanna read the book, I'll watch the movie.
Tekker's Lessons on GfB&B: Music Theory, Recording, and General Guitar
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